cryptococcus neoformans c neoformans h99 atcc  (ATCC)

Journal: Scientific Reports

Article Title: Standardisation of high throughput microdilution antifungal susceptibility testing for Candida albicans and Cryptococcus neoformans

doi: 10.1038/s41598-024-74068-2

Figure Lengend Snippet: MIC (µg/mL) of tested antifungals against C. Neoformans var. Grubii (H99) ATCC 208821 in 384-well NBS plates, by OD 570 − 600 readout with resazurin ( n = 4). # amphotericin B prepared from solution stock, * literature MICs for 5-flucytosine against C. neoformans are not strain specific.

Article Snippet: The MIC of ketoconazole against C. neoformans ATCC 208821 H99 in YNB were higher than the reference MIC.

Techniques: Inhibition

Journal: PLOS ONE

Article Title: Novel combinatorial approach: Harnessing HIV protease inhibitors to enhance amphotericin B’s antifungal efficacy in cryptococcosis

doi: 10.1371/journal.pone.0308216

Figure Lengend Snippet: Cultures of C . neoformans H99 were individually treated with different PIs (16 μg/mL) in the presence of AmB (0.0625 μg/mL). The growth intensity of treated cultures was measured spectrophotometrically at 540 nm. Ritonavir (RTV), lopinavir (LPV), atazanavir (ATV), saquinavir (SQV), and nelfinavir (NLF) were identified as hit compounds reducing the growth of H99 below the cut-off value (20% relative to control growth) in the presence of AmB. Other PIs including amprenavir (AMP), indinavir (IND), darunavir (DRV), and tipranavir (TIP) were less effective.

Article Snippet: C . neoformans H99 ATCC-208821 , Isolated from a patient with Hodgkin’s disease, NY, USA. , 0.25 , >128 , >128 , >128 , >128 , >128.

Techniques: Control

Journal: PLOS ONE

Article Title: Novel combinatorial approach: Harnessing HIV protease inhibitors to enhance amphotericin B’s antifungal efficacy in cryptococcosis

doi: 10.1371/journal.pone.0308216

Figure Lengend Snippet: Cultures of C . neoformans H99 were individually treated with PIs; including ritonavir (RTV), lopinavir (LPV), atazanavir (ATV), saquinavir (SQV) and nelfinavir (NLF) at 8 μg/mL; alone or in combination with AmB (0.125 μg/mL). The growth of treated cultures was evaluated for 48 hrs. The fungicidal activity was defined as ≥ 3 log10 CFU/mL reduction compared to the untreated control (DMSO).

Article Snippet: C . neoformans H99 ATCC-208821 , Isolated from a patient with Hodgkin’s disease, NY, USA. , 0.25 , >128 , >128 , >128 , >128 , >128.

Techniques: Activity Assay, Control

Journal: PLOS ONE

Article Title: Novel combinatorial approach: Harnessing HIV protease inhibitors to enhance amphotericin B’s antifungal efficacy in cryptococcosis

doi: 10.1371/journal.pone.0308216

Figure Lengend Snippet: The effect of protease inhibitors on PAFE of amphotericin B on C . neoformans H99.

Article Snippet: C . neoformans H99 ATCC-208821 , Isolated from a patient with Hodgkin’s disease, NY, USA. , 0.25 , >128 , >128 , >128 , >128 , >128.

Techniques:

Journal: PLOS ONE

Article Title: Novel combinatorial approach: Harnessing HIV protease inhibitors to enhance amphotericin B’s antifungal efficacy in cryptococcosis

doi: 10.1371/journal.pone.0308216

Figure Lengend Snippet: L4-stage larvae infected with C . neoformans H99 strain were treated with AmB alone (0.125 μg/mL) or combined with PIs (8 μg/mL). Untreated worms were involved as a negative control. The fungal burden of treated worms was evaluated 24 hrs following the treatment. Asterisks indicate a statistical significance ( P value < 0.05, as determined by one-way analysis of variance [ANOVA] using Dunnett’s test for multiple comparisons) compared to the untreated control.

Article Snippet: C . neoformans H99 ATCC-208821 , Isolated from a patient with Hodgkin’s disease, NY, USA. , 0.25 , >128 , >128 , >128 , >128 , >128.

Techniques: Infection, Negative Control, Control